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新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜
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Bioengineering
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JoVE Journal Bioengineering
A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
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新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜

DOI:
10.3791/51873-v

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13:30 min

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September 16, 2014

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, ,
1Department of Mechanical Science and Engineering,University of Illinois at Urbana-Champaign

Chapters

  • 00:05Title
  • 01:42Functionalizing the Top Glass Cover Slips
  • 03:18Preparing Polyacrylamide Gel Directly on Glass Bottom Petri Dishes
  • 06:31Functionalizing Polyacrylamide Gel with Fibronectin
  • 07:47Traction Force Experiments
  • 10:22Results: Localizing Fluorescent Microspheres within Polyacrylamide Gels for Traction Force Microscopy
  • 12:51Conclusion

Summary

Automatic Translation

Automatic Translation

制造含有荧光探针聚丙烯酰胺（PA）的凝胶传统技术涉及的夹附着表面和玻璃载片之间的硅胶。这里，我们表明，涂敷该滑动用聚-D-赖氨酸（PDL）和荧光探针的局部化的探针内1.6微米的凝胶表面。

Tags

Traction Force MicroscopyPA GelsFluorescent BeadsCell Traction ForcesSurface DeformationElasticity TheoryCell AdhesionExtracellular MatrixSurface DisplacementBead LocalizationPoly-D-lysineGel Fabrication

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