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A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
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Chapters
- 00:05Title
- 01:42Functionalizing the Top Glass Cover Slips
- 03:18Preparing Polyacrylamide Gel Directly on Glass Bottom Petri Dishes
- 06:31Functionalizing Polyacrylamide Gel with Fibronectin
- 07:47Traction Force Experiments
- 10:22Results: Localizing Fluorescent Microspheres within Polyacrylamide Gels for Traction Force Microscopy
- 12:51Conclusion
Traditional techniques for fabricating polyacrylamide (PA) gels containing fluorescent probes involve sandwiching a gel between an adherent surface and a glass slide. Here, we show that coating this slide with poly-D-lysine (PDL) and fluorescent probes localizes the probes to within 1.6 µm from the gel surface.
