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新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜
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Bioengineering
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Bioengineering
A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜
DOI:
10.3791/51873-v
•
13:30 min
•
September 16, 2014
•
Samantha G. Knoll
,
M. Yakut Ali
,
M. Taher A. Saif
1
Department of Mechanical Science and Engineering
,
University of Illinois at Urbana-Champaign
Chapters
00:05
Title
01:42
Functionalizing the Top Glass Cover Slips
03:18
Preparing Polyacrylamide Gel Directly on Glass Bottom Petri Dishes
06:31
Functionalizing Polyacrylamide Gel with Fibronectin
07:47
Traction Force Experiments
10:22
Results: Localizing Fluorescent Microspheres within Polyacrylamide Gels for Traction Force Microscopy
12:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
制造含有荧光探针聚丙烯酰胺(PA)的凝胶传统技术涉及的夹附着表面和玻璃载片之间的硅胶。这里,我们表明,涂敷该滑动用聚-D-赖氨酸(PDL)和荧光探针的局部化的探针内1.6微米的凝胶表面。
Tags
Traction Force Microscopy
PA Gels
Fluorescent Beads
Cell Traction Forces
Surface Deformation
Elasticity Theory
Cell Adhesion
Extracellular Matrix
Surface Displacement
Bead Localization
Poly-D-lysine
Gel Fabrication
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