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Neuroscience
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突触质膜和突触后密度蛋白采用不连续蔗糖梯度准备
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
Please note that all translations are automatically generated.
Click here for the English version.
突触质膜和突触后密度蛋白采用不连续蔗糖梯度准备
DOI:
10.3791/51896-v
•
08:06 min
•
September 03, 2014
•
Marie Kristel Bermejo*
1
,
Marija Milenkovic*
1
,
Ali Salahpour
,
Amy J. Ramsey
1
Department of Pharmacology and Toxicology
,
University of Toronto
Chapters
00:05
Title
01:16
Tissue Homogenization and Fractioning
03:57
Isolating the Synaptic Plasma Membrane (SPM) Fraction
07:02
Western Blot of Fractions
07:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文将详细介绍与突触质膜上超速不连续蔗糖梯度相关蛋白的富集。随后的制备突触后密度蛋白也被描述。蛋白制剂适用于免疫印迹或2D DIGE分析。
Tags
Synaptic Plasma Membrane
Postsynaptic Density
Sucrose Gradient
Neuronal Subcellular Fractionation
Synaptic Protein Isolation
Membrane Protein Isolation
Postsynaptic Density Protein Isolation
Western Blotting
2D DIGE
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