Rétroviral infection de cellules souches embryonnaires murines dérivées de cellules du corps embryoïdes pour l'analyse de la différenciation hématopoïétique
Manipulating temporal gene expression in differentiating embryonic stem cells (ESCs) can be achieved using inducible gene systems. However, generation of these cell lines is costly and time consuming. This protocol achieves rapid expression of a transgene in differentiating ES-derived cells and subsequent analysis of downstream hematopoietic differentiation.