Journal
/
Biology
/
代谢谱的实时分析<em>离体</em>鼠标肠隐窝类器官培养
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
Please note that all translations are automatically generated. Click here for the English version.

代谢谱的实时分析<em>离体</em>鼠标肠隐窝类器官培养

DOI:
10.3791/52026-v

•

08:53 min

•

November 03, 2014

•

,
1Department of Medicine,Albert Einstein College of Medicine, 2Department of Cell Biology,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 01:42Crypt Isolation and Culture
  • 06:17Extracellular Flux Assay
  • 07:36Results: Metabolism Assay Using Organoids Derived from 8 month old C57BL/6 Mice
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

小肠隐窝类器官培养离体提供的组织培养系统，该系统概括隐窝依赖于干细胞和它们的生态位的增长。我们建立了一个方法，以测定在实时在原代小​​鼠隐窝类器官的代谢分布图。我们发现组织体保持了其源定义的生理特性。

Tags

Intestinal Crypt OrganoidMouse IntestineMetabolic ProfileOxygen ConsumptionGlycolytic RateATP ProductionRespiratory CapacityReal-time AnalysisCrypt MicroenvironmentIntestinal Epithelial CellsAdult Stem CellsProgenitor CellsPaneth CellsStromal CellsImmune Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

真核Polyribosome剖面分析

活体成像和治疗卵巢癌的原位小鼠模型

斑马鱼胚胎的代谢谱分析

逆转录病毒感染的原发性肠化培养的视频协议

测量的刚度<em>离体</em>鼠标主动脉用原子力显微镜

小鼠小肠的混合原代培养拟肠激素分泌的研究和活细胞成像的肠内分泌细胞的供

实时在体记录了拟南芥钙信号在使用荧光传感器的昆虫饲喂时

眼部微血管血管生成前维沃胆球状芽分析

类器官来源的上皮单层：临床相关的肠道屏障功能体外模型

使用实时细胞代谢通量分析仪监测成骨细胞生物能量学

Read Article