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代谢谱的实时分析<em>离体</em>鼠标肠隐窝类器官培养
JoVE Journal
Biology
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JoVE Journal Biology
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
DOI:

08:53 min

November 03, 2014

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Chapters

  • 00:05Title
  • 01:42Crypt Isolation and Culture
  • 06:17Extracellular Flux Assay
  • 07:36Results: Metabolism Assay Using Organoids Derived from 8 month old C57BL/6 Mice
  • 08:20Conclusion

Summary

Automatic Translation

小肠隐窝类器官培养离体提供的组织培养系统,该系统概括隐窝依赖于干细胞和它们的生态位的增长。我们建立了一个方法,以测定在实时在原代小​​鼠隐窝类器官的代谢分布图。我们发现组织体保持了其源定义的生理特性。

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