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手册小分子屏幕接近高通量使用斑马鱼胚胎
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
DOI:

07:45 min

November 08, 2014

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Chapters

  • 00:05Title
  • 01:33Preparation of Zebrafish Mating Chambers and Collection of Embryos
  • 02:41Arraying and Chemically Treating Zebrafish Embryos
  • 04:34Embryo Fixation and Chemical Screening
  • 05:52Results: Zebrafish Embryonic High-throughput Chemical Screen
  • 07:17Conclusion

Summary

Automatic Translation

斑马鱼是一种极好的生物实验,研究脊椎动物发育过程和模型人类疾病。在这里,我们描述了如何用一个执行手动高通量化学屏幕在斑马鱼胚胎的协议整个安装原位杂交(WISH)读出。

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