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手册小分子屏幕接近高通量使用斑马鱼胚胎
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
手册小分子屏幕接近高通量使用斑马鱼胚胎
DOI:
10.3791/52063-v
•
07:45 min
•
November 08, 2014
•
Shahram Jevin Poureetezadi
,
Eric K. Donahue
,
Rebecca A. Wingert
1
Department of Biological Sciences
,
University of Notre Dame
Chapters
00:05
Title
01:33
Preparation of Zebrafish Mating Chambers and Collection of Embryos
02:41
Arraying and Chemically Treating Zebrafish Embryos
04:34
Embryo Fixation and Chemical Screening
05:52
Results: Zebrafish Embryonic High-throughput Chemical Screen
07:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
斑马鱼是一种极好的生物实验,研究脊椎动物发育过程和模型人类疾病。在这里,我们描述了如何用一个执行手动高通量化学屏幕在斑马鱼胚胎的协议整个安装
原位
杂交(WISH)读出。
Tags
Keywords: Zebrafish
Chemical Genetics
Small Molecules
High-throughput Screening
Whole-mount In Situ Hybridization
Developmental Biology
Disease Mechanisms
Translational Research
Therapeutic Compounds
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