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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
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Bioengineering
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JoVE Journal Bioengineering
Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

English

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10,424 Views

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08:23 min

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October 31, 2014

DOI:

10.3791/52186-v

DOI:
10.3791/52186-v

•

08:23 min
October 31, 2014

•

1 Views
, , ,
1Department of Biomedical Engineering,University of Wisconsin, Madison, 2Department of Mechanical Engineering,University of Wisconsin, Madison

Summary

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Cell culture substrates functionalized with microscale patterns of biological ligands have immense utility in the field of tissue engineering. Here, we demonstrate the versatile and automated manufacture of tissue culture substrates with multiple, micropatterned poly(ethylene glycol) brushes presenting orthogonal chemistries that enable spatially precise and site-specific immobilization of biological ligands.

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Culture SubstratesPoly(ethylene Glycol) (PEG) BrushesMicrocontact PrintingRobotic Microcontact Printing (R-μCP)Sequential Nucleophilic SubstitutionMicroscale PatterningCell-material InteractionsBiospecific Interactions

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Cite this Article

Knight, G. T., Klann, T., McNulty, J. D., Ashton, R. S. Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution. J. Vis. Exp. (92), e52186, doi:10.3791/52186 (2014).

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