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マウスの胚感覚軸索投影のホールマウントイメージング
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole-mount Imaging of Mouse Embryo Sensory Axon Projections
DOI:

08:37 min

December 09, 2014

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Chapters

  • 00:05Title
  • 01:23Tissue Preparation
  • 02:42Genotyping
  • 03:46Embryo Staining
  • 04:55Tissue Dehydration and Clearing
  • 05:53Whole Mount Imaging
  • 06:41Results: Analysis of Mouse Embryo Sensory Axon Projections Using Whole Mount Imaging
  • 07:56Conclusion

Summary

Automatic Translation

We present here an optimized protocol to genotype, stain and prepare fetal mice for the imaging of peripheral nociceptor axon projections in the whole animal, as an effective method to assess sensory axon growth phenotypes in developing genetically engineered mice.

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