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Developmental Biology
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胚様体の異なるサイズによって人工多能性幹(iPS)細胞から網膜色素上皮(RPE)の導出
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
Please note that all translations are automatically generated.
Click here for the English version.
胚様体の異なるサイズによって人工多能性幹(iPS)細胞から網膜色素上皮(RPE)の導出
DOI:
10.3791/52262-v
•
09:18 min
•
February 04, 2015
•
Alberto Muñiz
,
Kaini R. Ramesh
,
Whitney A. Greene
,
Jae-Hyek Choi
,
Heuy-Ching Wang
1
Ocular Trauma
,
U.S. Army Institute of Surgical Research
Chapters
00:05
Title
01:52
Induced Pluripotent Stem (iPS) Cell Culture and Passaging
03:39
Generation, Plating, and Differentiation of Embryoid Bodies (EBs)
06:28
Results: Representative Retinal Pigment Epithelium (RPE)
08:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
このレポートの目的は、胚様体の異なるサイズを使用して人工多能性幹(iPS)細胞から網膜色素上皮(RPE)を導出するためのプロトコルを記述することである。
Tags
Induced Pluripotent Stem Cells
Retinal Pigment Epithelium
Embryoid Bodies
Differentiation
Microwell Plates
Immunocytochemistry
RT-PCR
FACS Analysis
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