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基于ES-Cell的编程组合MYH6启动子,选择小鼠心脏起搏器细胞聚集一代
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
DOI:

08:52 min

February 17, 2015

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Chapters

  • 00:05Title
  • 01:19Differentiation Procedure
  • 04:56Selection of Sinus Nodal Cells
  • 07:12Results: Generation of Cardiac Pacemaker Cell Aggregates
  • 08:20Conclusion

Summary

Automatic Translation

本协议描述了如何制作从小鼠多能干细胞(PSC)功能性鼻窦淋巴组织。 T-BOX3(TBX3)表达以及心肌肌球蛋白重链(MYH6)启动抗生素的选择导致了高纯度的起搏细胞聚集。这些“诱导窦房结-机构”(“iSABs”)含有80%以上的起搏细胞,显示高度增加跳动的速度,并能步伐心肌体外

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