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蛋白质纯化技术，可以检测SUMO化修饰和芽殖酵母蛋白动粒和Ndc10泛素Ndc80的
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JoVE Journal Biology
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
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蛋白质纯化技术，可以检测SUMO化修饰和芽殖酵母蛋白动粒和Ndc10泛素Ndc80的

DOI:
10.3791/52482-v

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12:28 min

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May 03, 2015

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, ,
1Genetics Branch, Center for Cancer Research,National Cancer Institute, National Institute of Health

Chapters

  • 00:05Title
  • 01:48Extraction of Proteins
  • 04:54Purification of HF-Smt3 Conjugates
  • 07:31Immunoprecipitation of Myc-tagged Kinetochore Proteins
  • 09:52Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in S. cerisiae
  • 11:59Conclusion

Summary

Automatic Translation

Automatic Translation

此稿件描述了SUMO化修饰和着丝粒蛋白，Ndc10和Ndc80的泛素化的检测，在芽殖酵母 。

Tags

Keywords: Protein PurificationSumoylationUbiquitinationKinetochore ProteinsNdc10Ndc80Post-translational ModificationsSaccharomyces Cerevisiae

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