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Purificazione delle proteine ​​tecnica che permette la rilevazione di sumoilazione e Ubiquitinazione di lievito in erba cinetocore proteine ​​Ndc10 e Ndc80
JoVE Journal
Biology
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JoVE Journal Biology
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
DOI:

12:28 min

May 03, 2015

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Chapters

  • 00:05Title
  • 01:48Extraction of Proteins
  • 04:54Purification of HF-Smt3 Conjugates
  • 07:31Immunoprecipitation of Myc-tagged Kinetochore Proteins
  • 09:52Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in S. cerisiae
  • 11:59Conclusion

Summary

Automatic Translation

Questo manoscritto descrive la rilevazione di sumoilazione e ubiquitinazione delle proteine ​​cinetocore, Ndc10 e Ndc80, nel lievito Saccharomyces cerevisiae erba.

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