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Protein Purification teknikk som gjør at Påvisning av Sumoylation og ubiquitinering av Spirende gjær kinetochore Proteiner Ndc10 og Ndc80
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JoVE Journal Biology
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Protein Purification teknikk som gjør at Påvisning av Sumoylation og ubiquitinering av Spirende gjær kinetochore Proteiner Ndc10 og Ndc80

DOI:
10.3791/52482-v

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12:28 min

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May 03, 2015

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, ,
1Genetics Branch, Center for Cancer Research,National Cancer Institute, National Institute of Health

Chapters

  • 00:05Title
  • 01:48Extraction of Proteins
  • 04:54Purification of HF-Smt3 Conjugates
  • 07:31Immunoprecipitation of Myc-tagged Kinetochore Proteins
  • 09:52Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in S. cerisiae
  • 11:59Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskriptet beskriver påvisning av sumoylation og ubiquitinering av kinetochore proteiner, Ndc10 og Ndc80, i den spirende gjær Saccharomyces cerevisiae.

Tags

Keywords: Protein PurificationSumoylationUbiquitinationKinetochore ProteinsNdc10Ndc80Post-translational ModificationsSaccharomyces Cerevisiae

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