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蛋白质纯化技术,可以检测SUMO化修饰和芽殖酵母蛋白动粒和Ndc10泛素Ndc80的
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JoVE Journal
Biology
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Please note that all translations are automatically generated.
Click here for the English version.
蛋白质纯化技术,可以检测SUMO化修饰和芽殖酵母蛋白动粒和Ndc10泛素Ndc80的
DOI:
10.3791/52482-v
•
12:28 min
•
May 03, 2015
•
Kentaro Ohkuni
,
Yoshimitsu Takahashi
,
Munira A. Basrai
1
Genetics Branch, Center for Cancer Research
,
National Cancer Institute, National Institute of Health
Chapters
00:05
Title
01:48
Extraction of Proteins
04:54
Purification of HF-Smt3 Conjugates
07:31
Immunoprecipitation of Myc-tagged Kinetochore Proteins
09:52
Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in
S. cerisiae
11:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此稿件描述了SUMO化修饰和着丝粒蛋白,Ndc10和Ndc80的泛素化的检测,在芽殖
酵母
。
Tags
Protein Purification
Sumoylation
Ubiquitination
Kinetochore Proteins
Ndc10
Ndc80
Post-translational Modifications
Saccharomyces Cerevisiae
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