Journal
/
Biology
/
Proteinoprensning Teknik der tillader detektering af Sumoylation og Ubiquitinering af Spirende Gær Kinetochore Proteiner Ndc10 og Ndc80
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Proteinoprensning Teknik der tillader detektering af Sumoylation og Ubiquitinering af Spirende Gær Kinetochore Proteiner Ndc10 og Ndc80

DOI:
10.3791/52482-v

•

12:28 min

•

May 03, 2015

•

, ,
1Genetics Branch, Center for Cancer Research,National Cancer Institute, National Institute of Health

Chapters

  • 00:05Title
  • 01:48Extraction of Proteins
  • 04:54Purification of HF-Smt3 Conjugates
  • 07:31Immunoprecipitation of Myc-tagged Kinetochore Proteins
  • 09:52Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in S. cerisiae
  • 11:59Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver påvisning af sumoylation og ubiquitinering af kinetochore proteiner, Ndc10 og Ndc80, i spirende gæren Saccharomyces cerevisiae.

Tags

Protein PurificationSumoylationUbiquitinationKinetochore ProteinsNdc10Ndc80Post-translational ModificationsSaccharomyces Cerevisiae

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Measuring Replicative Life Span in the Budding Yeast

Detection of Protein Ubiquitination

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Purification of Mitochondria from Yeast Cells

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Purification of Hsp104, a Protein Disaggregase

Chromatografisk Rensning af højaktiv Gær ribosomer

Erhvervelse Fluorescens Time-lapse film fra Spirende Gær og analysere encellede Dynamics hjælp poder

Spirende Gær Protein ekstraktion og oprensning for Studiet af Function, Interactions og post-translationelle modifikationer

Grønt fluorescerende protein-baserede Expression Screening membranproteiner i<em> Escherichia coli</em

Read Article