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出芽酵母動原体タンパク質Ndc10とNdc80のSUMO化とユビキチン化の検出を可能にするタンパク質の精製技術
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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Please note that all translations are automatically generated.
Click here for the English version.
出芽酵母動原体タンパク質Ndc10とNdc80のSUMO化とユビキチン化の検出を可能にするタンパク質の精製技術
DOI:
10.3791/52482-v
•
12:28 min
•
May 03, 2015
•
Kentaro Ohkuni
,
Yoshimitsu Takahashi
,
Munira A. Basrai
1
Genetics Branch, Center for Cancer Research
,
National Cancer Institute, National Institute of Health
Chapters
00:05
Title
01:48
Extraction of Proteins
04:54
Purification of HF-Smt3 Conjugates
07:31
Immunoprecipitation of Myc-tagged Kinetochore Proteins
09:52
Results: Ndc80 and Ndc10 are Substrates for Sumoylation and Ubiquitination in
S. cerisiae
11:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この原稿は、出芽
酵母サッカロミセス·セレビシエ(Saccharomyces cerevisiae)
において、動原体タンパク質、Ndc10とNdc80のSUMO化とユビキチン化の検出について説明します。
Tags
Protein Purification
Sumoylation
Ubiquitination
Kinetochore Proteins
Ndc10
Ndc80
Post-translational Modifications
Saccharomyces Cerevisiae
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