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Immunology and Infection
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简化人力中性粒细胞胞外陷阱(英语教师)分离和处理
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
Please note that all translations are automatically generated.
Click here for the English version.
简化人力中性粒细胞胞外陷阱(英语教师)分离和处理
DOI:
10.3791/52687-v
•
09:43 min
•
April 16, 2015
•
Sara Najmeh*
1
,
Jonathan Cools-Lartigue*
1
,
Betty Giannias
,
Jonathan Spicer
,
Lorenzo E. Ferri
1
LD MacLean Surgical Research Laboratories, Department of Surgery
,
McGill University
Chapters
00:05
Title
01:14
Neutrophil Isolation from Whole Blood
03:38
Neutrophil Extracellular Trap (NET) Generation and Isolation
05:24
NET-cancer Cell Static Adhesion Assay
08:18
Results: Simple Generation and Isolation of NETs from Human Whole Blood
09:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在下面的协议中,我们描述了一个非常简单的方法来分离中性粒细胞外使用容易获得的试剂从人全血中的陷阱(母语)。然后,我们说明如何分离的母语可以与癌细胞
在体外
粘附试验中使用。
Tags
Neutrophil Extracellular Traps (NETs)
NET Isolation
NET Handling
Cell-free NET Isolation
NET-based Assays
NET Adhesion Assay
A549 Lung Cancer Cells
PMA Stimulation
Density Gradient Separation
DNase1
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