De dos fotones de imágenes de Ca intracelular<sup> 2 +</sup> Manejo y producción de óxido nítrico en el endotelio y las células del músculo liso de la aorta de rata aislada
Vascular cell functiondepends on activity of intracellular messengers. Described here is an ex vivo two photon imaging method that allows the measurement of intracellular calcium and nitric oxide levels in response to physiological and pharmacological stimuli in individual endothelial and smooth muscle cells of an isolated aorta.