Deux photons imagerie intracellulaire de Ca<sup> 2+</sup> Manipulation et production d'oxyde nitrique endothélial et en cellules de muscle lisse d'un Rat aorte isolée
Vascular cell functiondepends on activity of intracellular messengers. Described here is an ex vivo two photon imaging method that allows the measurement of intracellular calcium and nitric oxide levels in response to physiological and pharmacological stimuli in individual endothelial and smooth muscle cells of an isolated aorta.