A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:57Preparation of Media for C. elegans Growth
- 02:15Preparation for Gene Downregulation Using RNAi
- 03:50Preparation of Age-synchronized L4 Population
- 04:45Performing the Oxidative Stress Resistance Assay
- 06:43Results: flcn-1(ok975) Mutants are More Resistant than Wild-type C. elegans to Oxidative Stress
- 07:38Conclusion
C. elegans is an attractive model organism to study signal transduction pathways involved in oxidative stress resistance. Here we provide a protocol to measure oxidative stress resistance of C. elegans animals in liquid phase, using several oxidizing agents in 96 well plates.
