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成人人成纤维细胞的推导和他们直接转换成可扩展的神经祖细胞
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
DOI:

13:58 min

July 29, 2015

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Chapters

  • 00:05Title
  • 02:21Punch Biopsy
  • 06:12Infection of Human Fibroblasts with Sendai Virus and Transdifferentiation
  • 08:47Isolation of Putative Induced Neural Progenitor Cells (iNPC) Colonies
  • 10:15Differentiation of iNPCs
  • 12:04Results: Reprogramming of Fibroblasts into Neural Precursors and their Cellular Validation
  • 13:20Conclusion

Summary

Automatic Translation

诱导多能干细胞的产生提供了用于自体移植的推导引人入胜的前景。然而,通过多能状态和艰苦的再分化仍然进展阻碍临床翻译。在这里,我们描述了成年人成纤维细胞的衍生和其直接转化为诱导神经祖细胞和随后的分化成神经谱系。

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