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Applicazione di lungo termine colta interferone-γ Enzyme Linked immunospot Assay per la valutazione effettori e memoria delle cellule T risposte a Bovino
This content is Free Access.
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine,Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service,United States Department of Agriculture
Chapters
- 00:00Title
- 01:40Methods
- 02:05Long-term Culture
- 03:38Elispot Assay Day 1
- 04:30Elispot Assay Day 2
- 05:15Adherent Cell Isolation
- 05:37Flow Cytometry Plate Preparation
- 06:15Harvest the 13 Day Long-term Cells
- 09:05Elispot Assay Day 3
- 10:04Alkaline Phosphatase Preparation
- 11:08Substrate Solution Preparation
- 11:55Reading the Plate
- 12:12Performing Flow Cytometry
- 12:25Results
- 14:37Conclusions
A lungo termine colta interferone-γ enzimatico test immunospot viene usato come misura di risposte di memoria centrale e correla con le risposte vaccino anti-micobatteri protettivi. Con questo test, le cellule mononucleari del sangue periferico sono stimolati con antigeni micobatterici e interleuchina-2 per 14 giorni, differenziazione e consentendo l'espansione delle cellule T di memoria centrale.
