Application of Long-term cultured Interferon-&#947; Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Mayara F. Maggioli; Mitchell V. Palmer; H. Martin Vordermeier; Adam O. Whelan; James M. Fosse; Brian J. Nonnecke; W. Ray Waters

doi:10.3791/52833

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Aplicação de longo prazo cultivadas Interferon-γ Enzyme-linked Immunospot Ensaio para a Avaliação e efetoras de memória T respostas de células em gado

JoVE Journal
Immunology and Infection
Author Produced

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JoVE Journal Immunology and Infection

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Aplicação de longo prazo cultivadas Interferon-γ Enzyme-linked Immunospot Ensaio para a Avaliação e efetoras de memória T respostas de células em gado

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12,128 Views

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15:57 min

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July 11, 2015

DOI:

10.3791/52833-v

DOI:

10.3791/52833-v

•

15:57 min

July 11, 2015

•

4 Views

Mayara F. Maggioli^1,2, Mitchell V. Palmer¹, H. Martin Vordermeier³, Adam O. Whelan³, James M. Fosse⁴, Brian J. Nonnecke¹, W. Ray Waters¹

¹Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, ²Department of Veterinary Pathology, College of Veterinary Medicine,Iowa State University, ³UK Veterinary Laboratories Agency, ⁴Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service,United States Department of Agriculture

Summary

Ensaio enzyme-linked immunospot cultivadas interferão-γ longo termo é usado como uma medida das respostas de memória central e correlaciona-se com a resposta à vacina anti-micobacterianos de protecção. Com este ensaio, as células mononucleares de sangue periférico são estimuladas com antigénios micobacterianos e interleucina-2 durante 14 dias, permitindo a diferenciação e a expansão das células T de memória central.