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Immunology and Infection
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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Generation and Multi-phenotypic High-content Screening of
Coxiella burnetii
Transposon Mutants
Please note that all translations are automatically generated.
Click here for the English version.
生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体
DOI:
10.3791/52851-v
•
11:44 min
•
May 13, 2015
•
Eric Martinez
,
Franck Cantet
,
Matteo Bonazzi
1
Cell Biology of Bacterial Infections, CNRS, FRE3689, CPBS
,
Université Montpellier
Chapters
00:05
Title
01:49
Isolation of Individual Transposon Mutants
03:44
Evaluation of Bacterial Concentration and Single Primer Colony PCR
05:34
Eukaryotic Cell Challenge with
Coxiella
Mutants and Monitoring of Intracellular Growth
07:17
Preparation of Samples for Automated Image Acquisition
09:25
Results: Viability and Infectivity of
Coxiella
Transposon Mutants
11:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
贝氏柯克斯体
是专性细胞内革兰阴性细菌负责对人畜共患病Q热。在这里,我们描述了用于
Coxiella
荧光转座子突变体的产生,以及自动识别和所得内化,复制和细胞毒性表型分析的方法。
Tags
Coxiella Burnetii
Transposon Mutagenesis
GFP-tagged Mutant Library
High-content Screening
Host-pathogen Interactions
Virulence Factors
Q Fever
Intracellular Bacterial Pathogen
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