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生物膜制作的溶剂辅助脂质双分子层（SALB）方法

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Please note that all translations are automatically generated. Click here for the English version.

生物膜制作的溶剂辅助脂质双分子层（SALB）方法

DOI:

10.3791/53073-v

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09:38 min

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December 01, 2015

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Seyed R. Tabaei², Joshua A. Jackman², Minchul Kim², Saziye Yorulmaz², Setareh Vafaei², Nam-Joon Cho^2,3

¹School of Materials Science and Engineering,Nanyang Technological University, ²Centre for Biomimetic Sensor Science,Nanyang Technological University, ³School of Chemical and Biomedical Engineering,Nanyang Technological University

Chapters

00:05Title
01:22Formation of Supported Lipid Bilayer on a Hydrophilic Solid Support and Cholesterol-enriched Supported Membranes
03:05Membrane Fluidity Assay
05:01Cholesterol Quantification Assay
07:51Results: Fabrication of Planar Bilayer on Solid Support Using SALB Method
08:44Conclusion

Summary

Automatic Translation

我们提出了一个试验性协议，形成对固体基质支持的脂质双层不使用脂质囊。我们展示了一个一步法，以形成具有各种生物应用胆固醇富集域上的二氧化硅和金脂质双层以及支持膜。

Tags

Biomembrane Fabrication Solvent-assisted Lipid Bilayer SALB Method Supported Lipid Bilayer SLB Vesicle Fusion Lipid Deposition Organic Solvent Hydrophilic Surface Cholesterol-rich Membranes

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