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Neuroscience
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构建基于细胞的神经递质荧光工程记者(CNiFERs)神经递质的光学检测<em>在体内</em
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters
In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
构建基于细胞的神经递质荧光工程记者(CNiFERs)神经递质的光学检测<em>在体内</em
DOI:
10.3791/53290-v
•
12:48 min
•
May 12, 2016
•
Emre Lacin
,
Arnaud Muller
,
Marian Fernando
,
David Kleinfeld
3
,
Paul A. Slesinger
1
Department of Neuroscience
,
Icahn School of Medicine at Mount Sinai
,
2
Department of Physics
,
University of California, San Diego
,
3
Section of Neurobiology
,
University of California, San Diego
Chapters
00:05
Title
00:56
Lentiviral Transduction of HEK293/TN-XXL/Gqi5 Cells
02:49
Preparation for FACS and Isolation of the Single CNiFER Clones
05:17
CNiFER Implantation into Mouse Cortex
07:52
In Vivo
Imaging of CNiFER Clones
10:42
Results: Examples of Flexstation Analysis, Agonist-induced FRET Response and Dose Response Curves for D2 CNiFER
12:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个协议,用于容量神经递质释放的光学检测创建基于细胞的神经递质荧光工程记者(CNiFERs)。
Tags
Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs)
Optical Detection
Neurotransmitters
In Vivo
HEK293 Cells
Lentivirus
FACS Analysis
Agonist Curve
GPCR
TNXXL
FRET
Fluorometric Plate Reader
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