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실시간으로 마취 마우스에 생체 내에 현미경으로 장간막 혈관에서 호중구와 단핵구 이미징
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
DOI:

09:28 min

November 16, 2015

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Chapters

  • 00:05Title
  • 01:00Bone Marrow Single Cell Suspension Preparation
  • 02:38Neutrophil Enrichment
  • 04:18Neutrophil Labeling
  • 05:29In vivo Measurement of Neutrophil and Monocyte Behavior
  • 07:44Results: Representative Intravital Neutrophil and Monocyte Imaging
  • 08:46Conclusion

Summary

Automatic Translation

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

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