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Transformation af Probiotic Gær og deres Recovery fra Mave Immune væv efter oral sonde i mus
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
DOI:

12:12 min

February 08, 2016

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Chapters

  • 00:05Title
  • 01:00Generate Survival Curves to Determine Needed Doses of UV Irradiation
  • 03:01Screening for Mutagenized Auxotrophic Yeast Strains and Yeast Transformation
  • 05:42Oral Gavage of Mice with Transformed Yeast
  • 07:50Harvest of Murine Peyer’s Patches and Isolation of Viable Yeast Colonies
  • 10:03Representative Results
  • 11:28Conclusion

Summary

Automatic Translation

Præsenteret her er en samlet beskrivelse af teknikker, der kan anvendes til at udvikle, transformere, administrere og teste heterologt protein ekspression af de probiotiske gæren Saccharomyces boulardii.

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