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CD146的分离<sup> +</sup>从大鼠肺组织驻地肺间质基质细胞
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Isolation of CD146
+
Resident Lung Mesenchymal Stromal Cells from Rat Lungs
Please note that all translations are automatically generated.
Click here for the English version.
CD146的分离<sup> +</sup>从大鼠肺组织驻地肺间质基质细胞
DOI:
10.3791/53782-v
•
09:47 min
•
June 17, 2016
•
Jennifer J.P. Collins
2
,
Marius A. Möbius
3,4
,
Bernard Thébaud
2,5
1
Sinclair Centre for Regenerative Medicine
,
Ottawa Hospital Research Institute
,
2
University of Ottawa
,
3
Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus
,
Technische Universität Dresden
,
4
DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD)
,
Technische Universität, Dresden
,
5
Children’s Hospital of Eastern Ontario Research Institute
Chapters
00:05
Title
00:56
Lung MSC Isolation
04:17
CD146
+
Lung MSC Selection
07:15
Results: Representative Density Gradient- and Bead-isolated CD146
+
MSC
08:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个协议描述了从大鼠获得原发性肺癌驻地间充质基质细胞,通过使用酶消化,密度梯度分离,塑料吸附和CD146
+
磁珠选择的隔离技术。
Tags
CD146+
Resident Lung Mesenchymal Stromal Cells
Rat Lungs
Enzymatic Digestion
Density Gradient Separation
Plastic Adherence
Bead Sorting
Pulmonary Disease
Immune Modulation
Trachea
Bronchi
CPDA Anticoagulant
PBS
Dulbecco’s PBS
Sodium Pyruvate
Glucose
Scalpel
Mincing
Enzyme Digestion
EDTA
FBS
Cell Strainer
Article
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