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Isolering af CD146<sup> +</sup> Resident Lung Mesenchymale stromacellers fra Rotte Lunger
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JoVE Journal Biology
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

Isolering af CD146<sup> +</sup> Resident Lung Mesenchymale stromacellers fra Rotte Lunger

DOI:
10.3791/53782-v

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09:47 min

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June 17, 2016

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, ,
1Sinclair Centre for Regenerative Medicine,Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus,Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD),Technische Universität, Dresden, 5Children’s Hospital of Eastern Ontario Research Institute

Chapters

  • 00:05Title
  • 00:56Lung MSC Isolation
  • 04:17CD146+ Lung MSC Selection
  • 07:15Results: Representative Density Gradient- and Bead-isolated CD146+ MSC
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en isolation teknik til opnåelse primære lunge residente mesenchymal stromaceller fra rotter, ved brug af enzymatisk fordøjelse, gradient separation massefylde, plast adhærens og CD146 + magnetiske perler markering.

Tags

CD146+Resident Lung Mesenchymal Stromal CellsRat LungsEnzymatic DigestionDensity Gradient SeparationPlastic AdherenceBead SortingPulmonary DiseaseImmune ModulationTracheaBronchiCPDA AnticoagulantPBSDulbecco’s PBSSodium PyruvateGlucoseScalpelMincingEnzyme DigestionEDTAFBSCell Strainer

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