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Chapters
- 00:05Title
- 01:13Preparation of Electro-competent Bacterial Cells for Transformation
- 02:33Electroporation of Bacterial Cells with Complementation Plasmids
- 03:39Transformation of AOH1 to Facilitate Functional Complementation Using L,L-diaminopimelate Aminotransferase
- 04:18Transformation of TKL-11 to Facilitate Functional Complementation Using UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate Ligase
- 05:12Transformation of Mutant Hx699 to Facilitate Functional Complementation of Hypomucoid Phenotype of Novosphingobium sp. Strain Hx699
- 06:02Results: Functional Complementation Analysis
- 07:44Conclusion
The validation of enzymatic activities involved in biochemical pathways can be elucidated using functional complementation analysis (FCA). Described in this manuscript is the FCA assay demonstrating the enzymatic activity of enzymes involved in the metabolism of amino acids, bacterial stringent response and bacterial peptidoglycan biosynthesis.
