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功能互补分析(FCA):实验室练习设计和实施,以补充生化途径的教学
JoVE Journal
Biology
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JoVE Journal Biology
Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
DOI:

09:27 min

June 24, 2016

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Chapters

  • 00:05Title
  • 01:13Preparation of Electro-competent Bacterial Cells for Transformation
  • 02:33Electroporation of Bacterial Cells with Complementation Plasmids
  • 03:39Transformation of AOH1 to Facilitate Functional Complementation Using L,L-diaminopimelate Aminotransferase
  • 04:18Transformation of TKL-11 to Facilitate Functional Complementation Using UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate Ligase
  • 05:12Transformation of Mutant Hx699 to Facilitate Functional Complementation of Hypomucoid Phenotype of Novosphingobium sp. Strain Hx699
  • 06:02Results: Functional Complementation Analysis
  • 07:44Conclusion

Summary

Automatic Translation

所涉及的生化途径的酶活性的确认可以使用功能性互补分析(FCA)阐明。描述在这个手稿是FCA测定表明参与氨基酸,细菌严格的响应和细菌的肽聚糖的生物合成的代谢酶的酶活性。

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