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功能互补分析(FCA):实验室练习设计和实施,以补充生化途径的教学
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Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
Please note that all translations are automatically generated.
Click here for the English version.
功能互补分析(FCA):实验室练习设计和实施,以补充生化途径的教学
DOI:
10.3791/53850-v
•
09:27 min
•
June 24, 2016
•
André O. Hudson
,
Taylor C.M. Harkness
,
Michael A. Savka
1
Thomas H. Gosnell School of Life Sciences
,
Rochester Institute of Technology
Chapters
00:05
Title
01:13
Preparation of Electro-competent Bacterial Cells for Transformation
02:33
Electroporation of Bacterial Cells with Complementation Plasmids
03:39
Transformation of AOH1 to Facilitate Functional Complementation Using L,L-diaminopimelate Aminotransferase
04:18
Transformation of TKL-11 to Facilitate Functional Complementation Using UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate Ligase
05:12
Transformation of Mutant
Hx699
to Facilitate Functional Complementation of Hypomucoid Phenotype of
Novosphingobium sp.
Strain
Hx699
06:02
Results: Functional Complementation Analysis
07:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
所涉及的生化途径的酶活性的确认可以使用功能性互补分析(FCA)阐明。描述在这个手稿是FCA测定表明参与氨基酸,细菌严格的响应和细菌的肽聚糖的生物合成的代谢酶的酶活性。
Tags
Functional Complementation Analysis
Biochemical Pathways
Enzyme Activity
Mutant Cell
Wild Type Phenotype
In Vivo Conditions
In Vitro Conditions
Uncharacterized Enzymes
Putative Functions
Bacterial Cells
Gene Cloning
Electroporation
Competent Cells
Recombinant Plasmid
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