/

/

TRUE基因沉默：一个七聚物型小导RNA库的筛选潜在的癌症治疗药

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

Please note that all translations are automatically generated. Click here for the English version.

TRUE基因沉默：一个七聚物型小导RNA库的筛选潜在的癌症治疗药

DOI:

10.3791/53879-v

•

09:18 min

•

June 02, 2016

•

Arisa Haino*¹, Tatsuya Ishikawa*¹, Mineaki Seki, Masayuki Nashimoto

¹Research Institute for Healthy Living,Niigata University of Pharmacy and Applied Life Sciences

Chapters

00:05Title
00:41Construction of a Heptamer-type sgRNA Library
04:26Screening of the sgRNA Library for Cell Viability
05:44Evaluation of Effective sgRNAs by Flow Cytometry
07:15Effects of Heptamer-type sgRNAs on Leukemia and Myeloma Cell Lines
08:41Conclusion

Summary

Automatic Translation

在这里，我们提出了一个协议为七聚物型因组库潜在的治疗药物抗血癌的筛查。

Tags

Keywords: TRUE Gene Silencing Small Guide RNA (sgRNA)Heptamer-type SgRNA Library TRNase ZL RNA-directed Gene Silencing Cancer Therapeutic Agents Blood Cancers Myeloma Leukemia Cell Viability Apoptosis Flow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

支气管Thermoplasty：严重哮喘的一种新的治疗方法

原位Xenografting人类荧光素酶标记的恶性外周神经鞘瘤细胞在体内测试候选治疗药物

交付的治疗药物通过侧脑室（ICV）和静脉（IV）注射液在小鼠

一个简单的指南螺杆颅内移植瘤小鼠的研究方法

基于DNA载体的RNA干扰在癌症研究基因功能

一种新的手术方法气管内注射生物活性剂在胎鼠模型

对黑色素瘤修饰符一个斑马鱼原地肿瘤模型的筛选

原位膀胱癌模型的基因传递研究

多路复用讯问癌症相关的信号转导在培养的细胞中的荧光素酶的筛选平台

注重形成：一个基于Cell的检测，以确定一个基因的致癌性

Read Article