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TRUE基因沉默:一个七聚物型小导RNA库的筛选潜在的癌症治疗药
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JoVE Journal
Medicine
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
Please note that all translations are automatically generated.
Click here for the English version.
TRUE基因沉默:一个七聚物型小导RNA库的筛选潜在的癌症治疗药
DOI:
10.3791/53879-v
•
09:18 min
•
June 02, 2016
•
Arisa Haino*
1
,
Tatsuya Ishikawa*
1
,
Mineaki Seki
,
Masayuki Nashimoto
1
Research Institute for Healthy Living
,
Niigata University of Pharmacy and Applied Life Sciences
Chapters
00:05
Title
00:41
Construction of a Heptamer-type sgRNA Library
04:26
Screening of the sgRNA Library for Cell Viability
05:44
Evaluation of Effective sgRNAs by Flow Cytometry
07:15
Effects of Heptamer-type sgRNAs on Leukemia and Myeloma Cell Lines
08:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个协议为七聚物型因组库潜在的治疗药物抗血癌的筛查。
Tags
TRUE Gene Silencing
Small Guide RNA (sgRNA)
Heptamer-type SgRNA Library
TRNase ZL
RNA-directed Gene Silencing
Cancer Therapeutic Agents
Blood Cancers
Myeloma
Leukemia
Cell Viability
Apoptosis
Flow Cytometry
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