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TRUE genet Slå: Screening av en Heptamer-type liten guide RNA bibliotek for potensielle kreft Terapeutiske midler
JoVE Journal
Medicine
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JoVE Journal Medicine
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
DOI:

09:18 min

June 02, 2016

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Chapters

  • 00:05Title
  • 00:41Construction of a Heptamer-type sgRNA Library
  • 04:26Screening of the sgRNA Library for Cell Viability
  • 05:44Evaluation of Effective sgRNAs by Flow Cytometry
  • 07:15Effects of Heptamer-type sgRNAs on Leukemia and Myeloma Cell Lines
  • 08:41Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for screening av en heptamer-type sgRNA bibliotek for potensielle terapeutiske legemidler mot blodkreft.

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