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从脑边缘和Mesocortical多巴胺奖赏网站继朴素的糖和脂肪摄入大鼠c-fos激活的同时检测
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JoVE Journal Neuroscience
Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats
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从脑边缘和Mesocortical多巴胺奖赏网站继朴素的糖和脂肪摄入大鼠c-fos激活的同时检测

DOI:
10.3791/53897-v

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08:07 min

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August 24, 2016

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, ,
1Behavioral and Cognitive Neuroscience Cluster, Psychology Doctoral Program, The Graduate Center,CUNY, New York, NY, 2Department of Psychology, Queens College,CUNY, Flushing, NY, 3Behavioral and Cognitive Neuroscience Cluster, Psychology Doctoral Program, The Graduate Center,CUNY, Flushing, NY

Chapters

  • 00:05Title
  • 00:54Testing Apparatus and Intake Procedures
  • 02:06Tissue Preparation
  • 03:22c-fos Staining Procedure
  • 05:29Determination of c-fos Immunoreactive Counts
  • 06:45Results: Actual Amygdala and Ventral Tegmental Area c-fox Activation Following Fats and Sugars
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

这项研究的目的是通过使用蜂窝的c-fos的激活来测量大鼠的脂肪和糖新颖摄入后多巴胺途径和终端站点同步变化划定一个可靠免疫组织学技术，以确定奖励相关的分布式脑网络。

Tags

C-Fos ActivationMesolimbicMesocorticalDopamineRewardSugarFatIngestionVTABrainMotivationPalatable StimuliBehavioral AssaysCellular ActivationStatistical AnalysisFood RestrictionSaccharineIntake TestBrain DissectionParaformaldehydeSucrose SolutionOlfactory Bulb

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