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Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays
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Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays

Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays

DOI:
10.3791/53972-v

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07:48 min

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June 18, 2016

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, , ,
1Biozentrum,University of Basel

Chapters

  • 00:05Title
  • 00:58Observe the Early Starvation Response of Dictyostelium discoideum by Time-lapse Microscopy
  • 02:14cAMP Pulsing of Dictyostelium discoideum Cells During Starvation
  • 03:54Induction of Dictyostelium discoideum Development Through Exposure to Conditioned Medium
  • 05:32Results: cAMP Pulses Rescue the Early Developmental Defect of corA- Dictyostelium discoideum
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

The social amoeba Dictyostelium discoideum undergoes a developmental transition into a multicellular organism when starved. The evolutionary conserved protein coronin A plays a crucial role in the initiation of development. Using aggregation assays as our main method, we aim to elucidate the role of coronin A in early development.

Tags

Keywords: Dictyostelium DiscoideumCoronin AEarly Starvation ResponseAggregation AssaysDevelopmental BiologySignaling PathwaysCell DensityCell AdherenceTime-lapse MicroscopyCAMP PulsesCell CountingCell SuspensionShaking IncubatorCentrifugationHemocytometer24-well PlatePeristaltic Pump

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