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在脑片全细胞膜片钳记录

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Whole-cell Patch-clamp Recordings in Brain Slices

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在脑片全细胞膜片钳记录

DOI:

10.3791/54024-v

•

07:23 min

•

June 15, 2016

•

Amir Segev, Francisco Garcia-Oscos, Saïd Kourrich

¹Department of Psychiatry,University of Texas Southwestern Medical Center

Chapters

00:05Title
00:59Slice Placement and Visualization
02:15Micropipette Placement and Positioning
03:45Final Approach, Seal Formation, and Obtaining the Whole-cell Configuration
05:56Results: Effect of Temperature, Series Resistance, and Input Resistance on the Waveform of the Electrical Signal
06:47Conclusion

Summary

Automatic Translation

这个协议描述用于执行全细胞膜片钳记录基本程序步骤。这种技术允许神经元的电行为的研究，并在大脑切片进行时，允许的各种神经元功能从仍在集成在相对保存良好脑电路的神经元的评估。

Tags

Whole-cell Patch-clamp Brain Slices Neuroscience Neuron Function In Vitro In Vivo Recording Chamber ACSF Micropipette Internal Solution Electrode Holder Micromanipulator

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