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Developmental Biology

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Mikroinjektion for Transgenese og Genome Redigering i Trepigget Sticklebacks
 
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Mikroinjektion for Transgenese og Genome Redigering i Trepigget Sticklebacks

Article doi: 10.3791/54055
May 13th, 2016

Summary May 13th, 2016

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Transgene manipulationer og genom-redigering er kritiske for funktionelt teste roller gener og cis reguleringsmyndigheder elementer. Her et detaljeret mikroinjektion protokol for generering af genomiske modifikationer (herunder Tol2-medierede fluorescerende reporter transgene konstruktioner, Talens og CRISPRs) præsenteres for emergente model fisk, den trepigget hundestejle.

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