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Generation af induceret-pluripotente stamceller Brug fibroblastlignende synoviocytter Isoleret fra leddene på leddegigt patienter
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
DOI:

09:31 min

October 16, 2016

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Chapters

  • 00:05Title
  • 00:59Fibroblast-like Synoviocyte (FLS) Isolation
  • 02:51FLS Maintenance and Expansion
  • 03:51FLS Reprogramming via Yamanaka Factors-encoded Lentivirus
  • 06:08Colony Picking
  • 07:09Results: Representative FLS-iPSC Analyses
  • 08:39Conclusion

Summary

Automatic Translation

Her beskriver vi en protokol til generering humane induceret pluripotente stamceller fra patient-afledt fibroblastlignende synoviocytter, ved anvendelse af en lentiviral system uden fødeceller.

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