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Generation af induceret-pluripotente stamceller Brug fibroblastlignende synoviocytter Isoleret fra leddene på leddegigt patienter
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

Generation af induceret-pluripotente stamceller Brug fibroblastlignende synoviocytter Isoleret fra leddene på leddegigt patienter

DOI:
10.3791/54072-v

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09:31 min

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October 16, 2016

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, , ,
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology,Seoul St. Mary’s Hospital, Republic of Korea, 2Division of Rheumatology, Department of Internal Medicine,Seoul St. Mary’s Hospital, Institute of Medical Science, Republic of Korea, 3College of Medicine,The Catholic University of Korea, Republic of Korea

Chapters

  • 00:05Title
  • 00:59Fibroblast-like Synoviocyte (FLS) Isolation
  • 02:51FLS Maintenance and Expansion
  • 03:51FLS Reprogramming via Yamanaka Factors-encoded Lentivirus
  • 06:08Colony Picking
  • 07:09Results: Representative FLS-iPSC Analyses
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en protokol til generering humane induceret pluripotente stamceller fra patient-afledt fibroblastlignende synoviocytter, ved anvendelse af en lentiviral system uden fødeceller.

Tags

Induced-pluripotent Stem CellsIPSCsFibroblast-like SynoviocytesRheumatoid ArthritisSynoviocyte CultureCollagenaseDMEMFBSCell ExpansionCell DissociationEDTA

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