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ومنصة ميكروفلويديك لعزل وحيدة الخلية عالية الإنتاجية والثقافة
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
DOI:

09:51 min

June 16, 2016

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Chapters

  • 00:05Title
  • 01:35PDMS Device Preparation for Single-cell Isolation
  • 04:48Single-cell Isolation and Clonal Culture
  • 06:54Replacing the Culture Medium
  • 08:06Results: High-throughput Single-cell Isolation
  • 09:00Conclusion

Summary

Automatic Translation

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

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