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Gravação induzida pela temperatura actividade neuronal através de monitoramento das alterações de cálcio no bulbo olfativo de<em> Xenopus laevis</em
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Chapters
- 00:05Title
- 00:55Electroporation
- 02:06Whole Mount Preparation
- 04:06Bolus Loading
- 05:42Odor Application and Temperature Experiments
- 07:08Image Processing of the Post-synaptic Recordings Using Activity Correlation Imaging
- 08:50Results: Bolus Loading and ACI Dissociate Thermosensitive and Chemosensitive Networks
- 10:16Conclusion
Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.
