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JoVE Journal
Biology

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完整的工作流程为组蛋白翻译后修饰的分析采用自下而上质谱法:从组蛋白提取数据分析
 
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完整的工作流程为组蛋白翻译后修饰的分析采用自下而上质谱法:从组蛋白提取数据分析

Article DOI: 10.3791/54112
May 17th, 2016

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Summary May 17th, 2016

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Click here for the English version.

这个协议概述了表征使用质谱(MS)组蛋白的翻译后修饰的完全集成的工作流程。该工作流包括使用纳米流液相色谱法和用于数据分析的指令的细胞培养物或组织中,组蛋白衍生和消化,MS分析组蛋白纯化。该协议被设计为内完成2 - 3天。

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