Biology
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Komplett arbeidsflyt for analyse av Histone posttranslasjonelle modifikasjoner hjelp Bottom-up massespektrometri: Fra Histone Utvinning til dataanalyse
Chapters
Summary May 17th, 2016
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Denne protokollen beskriver et fullstendig integrert arbeidsflyt for karakterisering av histon post-translasjonelle modifikasjoner ved bruk av massespektroskopi (MS). Arbeidsflyten inkluderer histone rensing fra cellekulturer eller vev, histon derivatisering og fordøyelse, MS analyse ved hjelp av nano-flow væskekromatografi og instruksjoner for dataanalyse. Protokollen er utformet for ferdigstillelse innen 2 - 3 dager.
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