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为患者组织甲基结合DNA序列捕获
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Genetics
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JoVE Journal
Genetics
Methyl-binding DNA capture Sequencing for Patient Tissues
Please note that all translations are automatically generated.
Click here for the English version.
为患者组织甲基结合DNA序列捕获
DOI:
10.3791/54131-v
•
08:40 min
•
October 31, 2016
•
Rohit R. Jadhav
,
Yao V. Wang
,
Ya-Ting Hsu
,
Joseph Liu
,
Dawn Garcia
,
Zhao Lai
,
Tim H. M. Huang
,
Victor X. Jin
1
Department of Molecular Medicine
,
University of Texas Health Science Center at San Antonio
,
2
Greehey Children’s Cancer Research Institute
,
University of Texas Health Science Center at San Antonio
Chapters
00:05
Title
01:18
Methyl-binding DNA Capture
02:10
Single Fraction Elution
03:08
Ethanol Precipitation
04:29
Sequencing
05:58
Results: Sequencing of Methyl-binding DNA Capture
07:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,调查使用甲基结合DNA测序捕获(MBDCap-SEQ或MBD-SEQ)技术以及随后的生物信息学分析管道的大规模临床病人的筛查研究全基因组DNA甲基化。
Tags
DNA Methylation
Epigenetics
Patient Cohorts
Biomarkers
Methyl-binding DNA Capture
Sequencing
Genome-wide Analysis
Cost-effective
Sonication
Fragment Size
Elution
Magnetic Beads
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