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The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions
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JoVE Journal Neuroscience
The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

DOI:
10.3791/54186-v

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06:01 min

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August 11, 2016

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, ,
1Carilion Research Institute,Virginia Tech, 2Carilion School of Medicine,Virginia Tech, 3Department of Biological Sciences,Virginia Tech

Chapters

  • 00:05Title
  • 00:48Surgical Procedure
  • 03:31Results: Fibular Nerve Injury is a Reliable Assay for Identifying Neuromuscular Junction Repair Factors
  • 05:12Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a nerve injury method to reliably examine muscle reinnervation, and thus regeneration of neuromuscular junctions in mice. This technique involves injuring the common fibular nerve via a simple and highly reproducible surgery. Muscle reinnervation in then assessed by whole-mounting the extensor digitorum longus muscle.

Tags

Keyword Extraction: Fibular Nerve InjuryNeuromuscular JunctionsNerve RegenerationPeripheral Nerve InjuryNerve CrushNerve Injury MethodMouse ModelSurgical ProcedureNerve TracingMuscle SeparationNerve ExposureNerve Crush Injury

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