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该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克
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JoVE Journal Cancer Research
The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
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该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

DOI:
10.3791/54585-v

•

10:31 min

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October 06, 2016

•

,
1Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne

Chapters

  • 00:05Title
  • 01:16Genetic Crosses and Collection of Third Instar Larvae
  • 03:10Dissection of Larvae
  • 04:52Fixing and Staining of Tissues
  • 05:58Tissue Mounting and Quantification of Tumor Invasiveness
  • 07:10Results: Visualization, Tumor Quantification, and Gene Expression
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

该协议将演示如何生成的果蝇眼睛/触角成虫盘（EAD）荧光标记，基因定义克隆肿瘤。它介绍了如何从第三龄幼虫，以及如何处理它们的可视化和量化基因表达的变化和肿瘤的侵袭性解剖EAD和大脑。

Tags

DrosophilaImaginal DiscTumor ModelGenetic MosaicsGene ExpressionCell InvasivenessQuantificationFluorescent LabelingMARCMFLP-FRT SystemDissectionImmunostainingImage Analysis

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