A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:34Kinetic Assay for Quantification of Endocytosis of Cargos that Undergo Regulated Endocytosis
- 03:17Post-acquisition Analysis
- 05:15Population Analysis of Mup1-pHluorin Endocytosis by Flow Cytometry
- 07:37Results: Quantitative, Kinetic, and High-throughput Analysis of Endocytosis of pHluorin-tagged Proteins
- 09:03Conclusion
Accurate quantification of vesicular trafficking events often provides key insights into roles for specific proteins and the effects of mutations. This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry.
