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Assays für die Untersuchung der Rolle von Vitronectin in bakterielle Adhäsion und Serum-Widerstand
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
DOI:

10:55 min

October 16, 2018

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Chapters

  • 00:04Title
  • 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
  • 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
  • 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
  • 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
  • 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
  • 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
  • 08:29Results: Interaction of Vitronectin and Bacteria
  • 10:10Conclusion

Summary

Automatic Translation

Dieser Bericht beschreibt die Protokolle für die Charakterisierung von Interaktionen zwischen bakteriellen äußeren Membranproteine und menschliche Ergänzung Regler Vitronectin. Die Protokolle können verwendet werden, um die Bindung Reaktionen und die biologische Funktion von Vitronectin in alle Bakterienarten zu studieren.

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