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التصور من الفحم آجار ريسازورين الفحص لشبه الكمي، متوسطة الإنتاجية تعداد مايكوباكتيريا
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
DOI:

09:57 min

December 14, 2016

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Chapters

  • 00:05Title
  • 00:46Preparation of CARA Microplates
  • 02:20Setting up Replicating and Non-replicating MIC90 Assays
  • 04:23Inoculation of CARA Microplates
  • 05:30Developing CARA Microplates and Data Analysis
  • 07:22Results: Enumeration of Mycobacteria via the CARA Assay
  • 09:06Conclusion

Summary

Automatic Translation

The charcoal agar resazurin assay (CARA) is a semi-quantitative, medium-throughput method to assess activity of test agents against mycobacteria that are replicating, non-replicating, or both. The CARA permits rapid evaluation of time- and concentration-dependent activity and identifies parameters to pursue by colony forming unit (CFU) assays.

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